英语翻译After the membranes were blocked in 5% skimmed milk in P

英语翻译
After the membranes were blocked in 5% skimmed milk in PBS-T containing 0.05% Tween 20 for 2 h at RT,they were incubated with antibodies to mTOR,p70s6K,p-P70s6K,4EBP1,p-4EBP1 and b-actin diluted in 1% skimmed milk in PBS-T,respectively,at RT for 2 h,.