从SIGMA 买的T6884-100UN要怎么稀释?用什么Buffer?用多少Buffer稀释到多大浓度合适?

我不太懂生物的东西.不过也经常用Sigma的产品.以下是他们的官网对这一款凝血酶的使用说明,希望对你有所帮助.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs.Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
参考：
R.Biggs,ed.Human Blood Coagulation,Haemostasis and Thrombosis 2nd ed.,Philadelphia ,(1976),722

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