英语翻译摘要哺乳细胞基因组靶向修饰是生物技术、基础研究和基因治疗的重要方法.例如,通过同源重组靶向修饰小鼠胚胎干细胞的R

In mammalian cells targeted genome modification is biotechnology of the important methods for basic research and gene therapy For example,by homologous recombination targeted ROSA26 gene modified mice embryonic stem cells,has been widely used to obtain specific and stable transgenic mice However,the traditional transgenic technology can not work well in other cell type Zinc finger nuclease (any Zinc finger nucleases) technology,simplifies the cells of many species and gene targeting But,get effective specific zinc finger nuclease method has always been the technology difficulty We use the module assembly (Model) to an assembly method to build the targeted modified zinc finger nuclease ROSA26 gene The zinc finger nuclease activity depends on the module refers to the number of zinc in zinc finger nuclease in different cell amount of time and the enzyme activity and function of the zinc finger nuclease mediated ROSA26 locus gene type,improve the exogenous gene in efficient and specific expression in cells,the modified version of this extraordinary expression between cloning cells have a stronger ability to repeat these