请大家帮忙解决下,“以质粒载体pBR322为例,说明克隆载体的特点”此题答案是怎样的啊?

tybxjlx 1年前 已收到1个回答 举报

小城青年 幼苗

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除了质粒都有的复制序列外.克隆载体一般要有:
1. 酶切插入位点(该质粒多达40个)
2. 筛选基因(抗药基因),该质粒有2个(amp, tet).
FYI
pBR322 is a plasmid and for a time was one of the most commonly used E. coli cloning vectors. Created in 1977, it was named eponymously after its Mexican creators, p standing for plasmid, and BR for Bolivar and Rodriguez.

1年前

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