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Leonardo Da Vinci, a giant in the Italian Renaissance, is a distinguished painter as well as a sculptor and architector in human history, possessing the characteristics of both as an outstanding artist and natural scientist. He left footprint in every discipline as an all-round developed person.This project is centered on Da Vinci's aesthetic thought to discuss whether his aesthetic system exists and through an analysis of his theoretical work Treatise on Painting(书名用斜体)to explore how he embodied his aesthetic thought in the work in three aspects, humanism on beauty, scientific and natural scientific perspectives.