组培 ：ZT,6-BA,IBA,NAA,2-4D,的配制方法吗?

具体方法：
1 mg/5 mL BAP（就是6BA） stock: per 100 mL total solution, take 20 mg of BAP, wet with a
few mL of 0.1 M NaOH in a weigh boat, and place a few mL of 0.1 M NaOH into
the beaker. Wash wetted powder into the beaker to about 50% final volume.
Stir over heat to dissolve but do not boil. Place beaker at 4°C until cool and then
bring up to total volume. Do not adjust pH or autoclave. Store at 4°C.
1mg/5 mL 2,4-D stock: follow the same preparation and storage conditions as
for BAP stock.
1 mg/5 mL IBA stock: follow the same preparation and storage conditions as for
BAP stock.
1 mg/5 mL Zeatin stock: follow the same preparation and storage conditions as
for BAP stock.
方法其实都差不多,根据你需要的浓度换算后配就可以了.用水定容时,可以配完后过滤灭菌,也可以直接用无菌水配,这样就不用过滤灭菌了.