彩信和智恩
幼苗
共回答了17个问题采纳率:82.4% 举报
In recent years, oil pollution is serious day by day, how the remediation of contaminated soil and its method of research is increasingly becoming the focus of attention of everybody, and efficient hydrocarbon degradation bacterium screening is among them one of the favourites.Based on the laboratory preserved 3 of petroleum hydrocarbon degrading bacteria (Z1, Z41, Z49) preliminary determination, choose oil degradation effect is comparatively good strain, then in order to further improve the strains petroleum degradation efficiency, the ultraviolet mutation breeding, mutation time is divided into 5S, 15s, 30s, 40s, 60s blood plate, through screening and shaker rescreening, pick out the degradation of high efficiency strains, the final strain V23 degradation rate was 42.07%, and had increased by 20.07%, on several candidate strains to produce surface activity can evaluate, found that the strain of the surface active agent production also increased substantially, among them V23 2 μ L 10 times diluted supernatant oil ring reaches 37.14mm, rhamnolipid content reached to 19.6g/L, compared with the original strain improves more than 31%.
1年前
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