英语翻译用抗大鼠COX-2单抗包被于酶标板上,标准品和样品中的COX-2与单抗结合,加入生物素化的抗大鼠COX-2,形成

Coated ELISA plates with COX-2 anti-rat monoclonal antibody,then add biotinylated anti-rat COX-2 after the standard and samples of COX-2 combined with the monoclonal antibody.And the Streptavidin which marked by Horse radish peroxide enzyme combined with biotin.Then joins substrate driving fluid obviously blue color.Finally,add the termination of liquid sulfuric acid,measure the value of OD at 450nm,then draw the standard curve in a coordinate system which useing
standard 20,10,5,2.5,1.25,0.625,0.312,0 ng / ml for the abscissa,OD value for longitudinal coordinates.
And then,identify the corresponding COX-2 content.