英语翻译Fig.3.Truncated elongation on Nf wild-type and mutated s
英语翻译
Fig.3.Truncated elongation on Nf wild-type and mutated single-stranded oligonucleotide templates.The TP-linked DNA molecule resultant of replicating
ori(29)Nf in the presence of ddCTP is shown,assuming that the first and second nucleotides are recovered during elongation.Underlined T in the template
molecule marks the initiation site.Reactions were carried out in the presence of 2.3 x03M the indicated oligonucleotide,60 nM Nf DNA polymerase,120 nM Nf
TP,0.1 x03M the corresponding labeled dNTP,10 x03M each dATP,dGTP and dTTP,and 100 x03M ddCTP.Reactions were started by adding 1 mM MnCl2 and,after
30 min at 30 °C,were stopped and processed as indicated in Materials and Methods.In addition to the initiation complex (TP-dAMP or TP-dTMP),the position
corresponding to different end-products (TP-(dNMP)n) of each truncated elongation assay is indicated.
Fig.3.Truncated elongation on Nf wild-type and mutated single-stranded oligonucleotide templates.The TP-linked DNA molecule resultant of replicating
ori(29)Nf in the presence of ddCTP is shown,assuming that the first and second nucleotides are recovered during elongation.Underlined T in the template
molecule marks the initiation site.Reactions were carried out in the presence of 2.3 x03M the indicated oligonucleotide,60 nM Nf DNA polymerase,120 nM Nf
TP,0.1 x03M the corresponding labeled dNTP,10 x03M each dATP,dGTP and dTTP,and 100 x03M ddCTP.Reactions were started by adding 1 mM MnCl2 and,after
30 min at 30 °C,were stopped and processed as indicated in Materials and Methods.In addition to the initiation complex (TP-dAMP or TP-dTMP),the position
corresponding to different end-products (TP-(dNMP)n) of each truncated elongation assay is indicated.
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