英语翻译The sample digest was then purified until a minimal prot
英语翻译
The sample digest was then purified until a minimal protein
interface was obtained by a series of equal volume
extractions of 0.1 M Tris pH8 equilibrated phenol,phenol/chloroform/isoamylalcohol (24:24:1) and chloroform/isoamylalcohol (24:1).0.2 volumes of 10M ammonium acetate and 2 volumes of absolute ethanol
were added to precipitate the DNA from the final aqueous phase.
求专业 非专业人士请绕行！
The sample digest was then purified until a minimal protein
interface was obtained by a series of equal volume
extractions of 0.1 M Tris pH8 equilibrated phenol,phenol/chloroform/isoamylalcohol (24:24:1) and chloroform/isoamylalcohol (24:1).0.2 volumes of 10M ammonium acetate and 2 volumes of absolute ethanol
were added to precipitate the DNA from the final aqueous phase.
求专业 非专业人士请绕行！
赞 cracker227 花朵
共回答了27个问题采纳率：100% 举报
饭粒啊,看到好友动态,才知道你到这里来求翻译,以后直接找我就好了（翻译的时候我给你加了一些词,有助于理解）
（蛋白）消化后的样本用用以下步骤提纯,以尽量减少残留蛋白的干扰：
依次用等体积一下溶液萃取:
酚（事先用0.1M pH8 Tris 缓冲液平衡过,就是把酚和Tris混合震荡,然后离心分离得到）,再用酚：氯仿：异戊醇（24:24:1）混合液,最后是氯仿和异戊醇混合液（24:1）.
在最终（萃取得到）的DNA水溶液中加入0.2体积的10M浓度的醋酸铵和2体积的无水酒精,来使DNA沉淀出来.
（蛋白）消化后的样本用用以下步骤提纯,以尽量减少残留蛋白的干扰：
依次用等体积一下溶液萃取:
酚（事先用0.1M pH8 Tris 缓冲液平衡过,就是把酚和Tris混合震荡,然后离心分离得到）,再用酚：氯仿：异戊醇（24:24:1）混合液,最后是氯仿和异戊醇混合液（24:1）.
在最终（萃取得到）的DNA水溶液中加入0.2体积的10M浓度的醋酸铵和2体积的无水酒精,来使DNA沉淀出来.
1年前
6
可能相似的问题
你能帮帮他们吗