文献:Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression里
(E) CRISPRi regulation is reversible. Both dCas9 and sgRNA (NT1) are under the control of an aTc-inducible promoter. Cell culture was maintained during exponential phase. At time T = 0, 1 μM of aTc was supplemented to cells with OD = 0.001. Repression of target mRFP starts within 10 min. The fluorescence signal decays in a way that is consistent with cell growth, suggesting that the decay is due to cell division. In 240 min, the fluorescence reaches the fully repressed level. At T = 370 min, aTc is washed away from the growth media, and cells are diluted back to OD = 0.001. Fluorescence starts to increase after 50 min and takes about 300 min to rise to the same level as the positive control. Positive control: always without the inducer; negative control: always with 1 μM aTc inducer.
两个LOG(OD600)的小图,T与R代表的意思,还有negative control如何实现。这3个地方完全不明白,谢谢解答
破月弄影onlyme 幼苗
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你能帮帮他们吗
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