请问细胞生物学文献里的图谁能看得懂,帮我解释下。谢谢

请问细胞生物学文献里的图谁能看得懂,帮我解释下。谢谢

文献:Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression里

(E) CRISPRi regulation is reversible. Both dCas9 and sgRNA (NT1) are under the control of an aTc-inducible promoter. Cell culture was maintained during exponential phase. At time T = 0, 1 μM of aTc was supplemented to cells with OD = 0.001. Repression of target mRFP starts within 10 min. The fluorescence signal decays in a way that is consistent with cell growth, suggesting that the decay is due to cell division. In 240 min, the fluorescence reaches the fully repressed level. At T = 370 min, aTc is washed away from the growth media, and cells are diluted back to OD = 0.001. Fluorescence starts to increase after 50 min and takes about 300 min to rise to the same level as the positive control. Positive control: always without the inducer; negative control: always with 1 μM aTc inducer.

两个LOG(OD600)的小图,T与R代表的意思,还有negative control如何实现。这3个地方完全不明白,谢谢解答


划过寒冷 1年前 已收到2个回答 举报

破月弄影onlyme 幼苗

共回答了12个问题采纳率:91.7% 举报

T1/2指的是半衰期。
R²指的是时间和logOD值的相关性,越接近于1,相关性越大
阴性对照应该用的是没有启动子的但是含有RFP的序列。

1年前

1

mmlpp2008 幼苗

共回答了8个问题采纳率:75% 举报

布吉岛啊,。。。。

1年前

0
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