英语翻译Here,we report on the optimisation and validation of a l

英语翻译
Here,we report on the optimisation and validation of a liquid chromatographic method for the determination of 12 biologically active amines from
vegetal food products in a single 40-min run.The suitability of the method was checked in five vegetal products of distinct matrix:spinach (leaves),
hazelnut (high protein and fat content),banana,potato (high starch content),and milk chocolate (processed).Sample preparation consisted of a
0.6M perchloric acid extraction from a minced homogeneous aliquot.For samples with high starch content,a previous mild hydrolytic treatment
was required to prevent gel formation.The range of linearity was from 0.1 to 10 mg/l,except for serotonin and spermine (from 0.5 to 10 mg/l),
and the correlation coefficient was higher than 0.997 (P < 0.001) for all standard curves.The detection limits and the determination limit were
below 0.07 and 0.2 mg/l,respectively,except for spermine,which was 0.14 and 0.4 mg/l.The precision of the method was satisfactory; the relative
standard deviation obtained for each amine in each product was acceptable according to Horwitz.Recovery was between 77 and 110% for all
amines,irrespective of the product.