英语翻译Creamy friable calli were induced from meristems(scalps)
英语翻译
Creamy friable calli were induced from meristems
(scalps) of proliferating shoots of plantain (Musa sp.)
cv.Spambia (genome AAB) incubated on a semi-solid
modified Murashige and Skoog (MS) medium supplemented
with 4.5 lM 2,4-dichlorophenoxyacetic acid (2,4-
D) and 1.0 lM zeatin.About 25% of shoot-tip explants
formed scalps,and about 98% of scalps developed
embryogenic calli.Small dense aggregates of cells,were
obtained when these calli were transferred to liquid MS
medium supplemented with 4.5 lM 2,4-D and 1.0 lM
zeatin.Upon transfer to semi-solid MS medium of the same
composition as described above,aggregates of cells formed
somatic embryos.In the presence of 2.5 lM abscisic acid
(ABA),maturation of somatic embryos was 2.6-fold higher
than that of control (lacking ABA),and regardless of the
type of cytokinin used in the medium.Upon transfer to MS
medium supplemented with 1.25 lM 6-benzyladenine
(BA),80% of germinated embryos developed into plantlets.