分离纯化蛋白质如何除色素

cjsy43 1年前 已收到2个回答 举报

过客77757 幼苗

共回答了14个问题采纳率:78.6% 举报

If the pigment or color is from small molecules,you can pass the protein preparation through size column ( also called gel filtration).If you do not mind about the activity of the protein,you could use some solvents,such as acetone,to precipitate the protein,then centrifuge,and resuspend the protein in your desired solution.
You have to keep it in mind that some protein has pigment,such as hemoglobin,ceruloplasma and others.If you deprive those pigment or colored materials,the protein will not be active any more.
Good luck.

1年前

2

184609 幼苗

共回答了40个问题 举报

色素一般分子量比较小,用分子筛就可以除去了。

1年前

1
可能相似的问题
Copyright © 2024 YULUCN.COM - 雨露学习互助 - 17 q. 0.016 s. - webmaster@yulucn.com