英语翻译LDA was applied to evaluate which of these three ratios
英语翻译
LDA was applied to evaluate which of these three ratios provided the maximum discrimination (i.e.,best predictor) between the cell lines.(1,3,28,29) We applied LDA in a stepwise fashion.First,we applied LDA to each polymer separately,where we evaluated each potential peak value based on its ability to discriminate between (or predict) the four cell lines.Each of these analyses was conducted using 32 observations (4 cell lines × 8 replications) and 4 variables (the cell line indicator and the three emission intensity wavelength variables).This LDA analyses step identifies the optimal intensity of 410 nm for the P2 polymer and 420 nm for the P1 polymer.
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Other proteins in the conditioned media are interacting with the polymers,causing the observed intensity changes in the emission spectra.While we do not have an explanation yet for the observed pattern of emission intensity changes,we reasoned that these differential modulations can be used to distinguish the cancer cells.In this endeavor,we calculated the ratios of emission intensity (410,510,and 541 nm for P1; 420,520,and 541 nm for P2) in the presence of conditioned and unconditioned culture media (Table S-2,Supporting Information).These ratios were subsequently subjected to linear discriminant analysis (LDA).