fengxinzi1010
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In the production of luzhou-flavor liquor pits mud for wine production, the quality is very important. Pit mud in filamentous fungi produce amylase, protease, lipase, cellulase, hemicellulase and pectinase, tannin enzyme and esterification enzyme biolite affects metabolic product and liquor flavor. So, pit mud in filamentous fungi to improve the quality of pit mud and further clarify causal relationship of pit mud and liquor quality has important significance.
This study adopts the method of plate dilution. The filamentous fungi of pit mud culture, and then carries on the morphology, size, color, surface morphology, texture, spores, sac, Yin, Yin plexus) observation and physiological and biochemical (ferulic acid ethyl ester enzyme activity and amylase activity). Results there are 6 strains of bacteria have ferulic acid ethyl ester enzyme activity, 1 strain of amylase activity. Has chosen a strain of ferulic acid ethyl ester enzyme activity of high strain, after extraction of DNA for the PCR amplification, the amplification of 18 s sequencing to sequencing company, ITS sequence sequencing results are obtained. In NCBI Blast nucleic acid after removing vector sequence alignment, with a white silk gown mould ITS 99% sequence similarity, ultimately determine the filamentous fungi for white silk gown mould.
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1年前
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