英语翻译Ten iridoviruses were obtained from four different fish

英语翻译
Ten iridoviruses were obtained from four different fish cultured in Korea during
epidemics from 2000 to 2002 (Fig.1).Sources of the fish iridoviruses used in this
study are listed in Table 1.
The full-length ORF of the MCP gene was amplified from uncultured spleen DNA using polymerase chain reaction (PCR).Spleen from infected fish was
homogenized in a lysis buffer (4 M urea,200 mM Tris,20 mM NaCl,200 mM EDTA,pH 7.4).After centrifugation at 1700 g for 10 min,the supernatant was
treated with proteinase K (0.2 mg/ml) and sarcosyl (1%) at 45 .C for 3 h,followed by phenol/chloroform extraction and ethyl alcohol precipitation.The genomic
DNA in the pellet was dissolved in TE (10 mM Tris-HCl and 1 mM EDTA,pH 7.5) and used as a template for PCR.Primers for PCR were designed from nucleotide
sequences in the GenBank/EMBL databases of RBIV (AY532606,Do et al.[3]).PCR primers were as follows