漂渺小鱼
春芽
共回答了15个问题采纳率:100% 举报
您好,从您提出的问题来看,您似乎是想做一个动物模型(可能是炎症相关或损伤修复),然后选取了以上检测指标来比较实验组和对照组.但是您的问题让我有点困惑,比如NF-kb的激活通常是通过下游基因的表达来进行检测,另外免疫细胞和内皮细胞之间的调控是很纠结的,造模后单独分离出一种细胞进行检测的做法不太常见,至少我第一反应是做免疫组化.但可能是我误会了您的实验目的,所以如果可能的话,还望邮件或站内私信联系,谢谢!
1年前
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hchcfighting
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您好,我不做动物模型,只是从动物血液中通过提取mRNA,来看一下以上酶(主要是iNOS)的表达量,但是我不知道iNOSmRNA是存在于淋巴细胞还是单核细胞中,如果知道了是什么细胞分泌的iNOS,那我就知道了到底是提取哪种细胞的mRNA,还望解答,不胜感激!
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漂渺小鱼
您好,根据UCSC数据库,iNOS即NOS2(Nitric oxide synthase, inducible)在小鼠中主要由巨噬细胞(Macrophage)表达。以下信息可能对您有用: ID: NOS2_MOUSE DESCRIPTION: RecName: Full=Nitric oxide synthase, inducible; EC=1.14.13.39; AltName: Full=Inducible NO synthase; Short=Inducible NOS; Short=iNOS; FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. ENZYME REGULATION: Not stimulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. TISSUE SPECIFICITY: Macrophages. <----------您要的信息在这里 INDUCTION: By treatment with endotoxins or cytokines.