为什么相同分子量的线状、开环和超螺旋DNA在琼脂糖凝胶中移动速度是不一样?

为什么相同分子量的线状、开环和超螺旋DNA在琼脂糖凝胶中移动速度是不一样?
为什么超螺旋DNA移动最快,而线状双链DNA移动最慢?
whpit 1年前 已收到1个回答 举报

亿笙 种子

共回答了12个问题采纳率:91.7% 举报

"Supercoiled" (or "Covalently Closed-Circular") DNA is fully intact with both strands uncut, and with a twist built in, resulting in a compact form, so they move faster others."Linear" DNA has free ends, it moves at the speed similar to its molecular weight, compared to the markers."Nicked Open-Circular" DNA has one strand cut, it is not supercoiled, and it is not compact form, but it is circular. Therefore, it migrates slower than the above 2 forms. please see the picture.

1年前

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